Patch-seq
Patch-Clamp Electrophysiology Combined with Single-Cell RNA-seq
Patch-seq（ホールセル・パッチクランプ電気生理＋単一細胞RNA解析＋3D形態再構築）
1. Biological & Technical Mechanism
Patch-seq integrates whole-cell patch-clamp recording with intracellular dye biocytin filling and aspiration of the cell's cytoplasmic/nuclear contents for single-cell RNA sequencing. This bridges the historic divide between physiological/anatomical neuron classification and molecular transcriptomic cell-type taxonomies (MET-types: Morpho-Electro-Transcriptomic types).
Patch-seqは、脳スライス上の単一ニューロンに対してホールセル・パッチクランプ記録を行って膜電位応答・活動電位発火パターンを計測した後、記録ピペットからバイオサイチンを注入して3D形態を可視化し、さらに細胞内容物を吸引回収して単一細胞RNA-seqを実施する手法です。これにより、遺伝子発現（Transcriptome）・電気生理（Electrophysiology）・形態（Morphology）を統合した「MET細胞種分類」が可能になります。
2. Open Science & Clinical Trial Significance
Massive open Patch-seq datasets from mouse and human neurosurgical cortical tissues are hosted on the DANDI Archive in NWB format and cross-linked with the Allen Cell Types Database.
DANDI ArchiveおよびAllen Brain Mapでは、マウスおよびヒト大脳皮質の数千細胞規模のPatch-seqデータセットがNWB規格でオープン公開されており、特定の疾患関連イオンチャネル変異が発火特性に与える影響を検証する貴重な基盤となっています。
Related Neuroscience Terms & Standards
Unified data standard for cellular neurophysiology, packaging intracellular/extracellular recordings, optical physiology, and behavior into HDF5/Zarr files.
High-throughput transcriptomic profiling at individual cell or nucleus resolution, enabling identification of disease-associated microglia (DAM), astrocytes, and vulnerable neuronal subtypes.
High-temporal-resolution recording of postsynaptic cortical electrical potentials and neural oscillations (delta, theta, alpha, beta, gamma bands).
Open Datasets & Research Publications Related to Patch-seq
Dandiset 000108: Single-Cell Patch-seq Electrophysiology and Morphometry of Human Neocortical Neurons
Over 1,200 human surgical neocortical pyramidal neurons and interneurons characterized simultaneously with patch-clamp electrophysiology, fluorescent morphological reconstruction, and single-cell RNA sequencing in NWB 2.0 format.
Dandiset 001475: NHP patch-seq
BRAIN Initiative neurophysiology dataset deposited to DANDI Archive.
Dandiset 000108: Single-Cell Patch-seq Electrophysiology and Morphometry of Human Neocortical Neurons
Over 1,200 human surgical neocortical pyramidal neurons and interneurons characterized with patch-clamp electrophysiology, morphological reconstruction, and scRNA-seq.
Allen Brain Atlas: Human Brain ISH Autism Study
High-resolution ISH data detailing gene expression with a unique panel of lamina- and cell type- specific markers to phenotype cortical microstructure during the early years after autism onset.... Freely accessible open data product maintained by the Allen Institute for Brain Science.
BICCN Whole Human Brain Single-Cell Transcriptomic Cell Type Atlas
Cell atlas spanning >3 million single nuclei across 100+ human brain regions, mapping distinct vulnerable neuronal and glial sublineages.
Allen Brain Atlas: Visual Coding Optical Physiology
Time series of 2P in vivo neuronal responses to visual stimuli across Cre lines, higher visual areas and cortical layers... Freely accessible open data product maintained by the Allen Institute for Brain Science.
Disclaimer: This encyclopedia entry is intended for academic neuroscience research and data science reference only, and does not constitute medical advice.